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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
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Extracellular Vesicle Preparation and Analysis: A State-of-the-Art Review
Zesheng Wang1,2,3, Xiaoyu Zhou1,2,3, Qinglong Kong4
1Department of Precision Diagnostic and Therapeutic Technology, City University of Hong Kong Shenzhen Futian Research Institute, Shenzhen, Guangdong, 518000, P. R. China.
Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|June 14, 2024
Summary
Extracellular vesicles (EVs) are crucial in health and disease, but their complexity hinders clinical use. This review explores advanced preparation and analysis methods, including AI and sensors, to unlock their biomedical potential.
Area of Science:
- Life Sciences
- Biomedical Research
- Nanotechnology
Background:
- Extracellular vesicles (EVs) play vital roles in physiological and pathological processes.
- Their heterogeneity and complex origins present significant challenges for clinical translation.
- EVs show promise for disease diagnosis, drug delivery, and therapeutic applications.
Purpose of the Study:
- To provide a comprehensive overview of EV biogenesis, composition, and sources.
- To discuss contemporary and state-of-the-art techniques for EV preparation and analysis.
- To highlight innovative approaches and future research directions for EV applications.
Main Methods:
- Review of current literature on EV biogenesis and sources.
- Analysis of microfluidic and non-microfluidic platforms for EV processing.
- Exploration of artificial intelligence and electrochemical sensors for EV analysis, including single EV analysis.
Main Results:
- Detailed overview of EV characteristics and origins.
- Discussion of advanced EV preparation and analysis technologies.
- Identification of challenges and future research opportunities in the EV field.
Conclusions:
- Standardized and advanced methods are needed for EV preparation and analysis.
- Innovative technologies like AI and electrochemical sensors are crucial for single EV analysis.
- Further research is essential to fully realize the biomedical potential of extracellular vesicles.
