Related Experiment Video

Updated: Aug 16, 2026

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
11:19

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

Transcription signals in a region essential for replication of plasmid R6K

A Shafferman, D R Helinski

    Plasmid
    |January 1, 1985
    PubMed

    More Related Videos

    Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach11:12

    Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach

    Published on: September 11, 2017

    Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks09:07

    Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks

    Published on: September 20, 2021

    Related Concept Videos

    Bacterial RNA Polymerase00:43

    Bacterial RNA Polymerase

    Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
    In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
    Prokaryotic Transcriptional Activators and Repressors01:58

    Prokaryotic Transcriptional Activators and Repressors

    The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
    Transcription of prokaryotic...
    Transcription Attenuation in Prokaryotes02:42

    Transcription Attenuation in Prokaryotes

    Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
    There are several different mechanisms used to attenuate transcription. In ribosome mediated...
    Bacterial Transcription01:53

    Bacterial Transcription

    RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
    Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
    Transcription Initiation01:47

    Transcription Initiation

    Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
    The promoters and enhancers and their accessory proteins allow tight regulation of...
    Prokaryotic Transcriptional Activators and Repressors01:58

    Prokaryotic Transcriptional Activators and Repressors

    The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
    Transcription of prokaryotic...