Protocol for fiber photometry recording from deep brain regions in head-fixed mice
Siyao Zhou1, Shumin Duan1, Hongbin Yang1
1Department of Neurobiology and Department of Affiliated Mental Health Center of Hangzhou Seventh People's Hospital & Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou 310000, China; MOE Frontier Science Center for Brain Science & Brain-Machine Integration, State Key Laboratory of Brain-machine Intelligence, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou 310000, China; NHC and CAMS Key Laboratory of Medical Neurobiology, Zhejiang University, Hangzhou 310000, China.
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To exclude the influence of motion on in vivo calcium imaging, animals usually need to be fixed. However, the whole-body restraint can cause stress in animals, affecting experimental results. In addition, some brain regions are prone to bleeding during surgery, which lowers the success rate of calcium imaging. Here, we present a protocol for calcium imaging using heparin-treated fiber in head-fixed mice. We describe steps for stereotaxic surgery, including virus injection and optic fiber implantation, fiber photometry, and data analysis. For complete details on the use and execution of this protocol, please refer to Du et al.1.
