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DNA alterations in ovarian adult granulosa cell tumours: A scoping review protocol
Sven Karstensen1, Karsten Kaiser1, Caroline Moos2
1Department of Womens's Health, University of Southern Denmark, Sygehus Sønderjylland, Aabenraa, Denmark.
Background:
Identifying and describing molecular alterations in tumors has become common with the development of high-throughput sequencing. However, DNA sequencing in rare tumors, such as ovarian adult granulosa cell tumor (aGCT), often lacks statistical power due to the limited number of cases in each study. Questions regarding personalized treatment or prognostic biomarkers for recurrence or other malignancies therefore still need to be elucidated. This scoping review protocol aims to systematically map the current evidence and identify knowledge gaps regarding DNA alterations, actionable variations and prognostic biomarkers in aGCT.
Methods:
This scoping review will be conducted based on Arksey and O'Malley's methodological framework and later modifications by JBI Evidence Synthesis. The protocol complies with Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for scoping reviews. All original publications describing molecular alterations of aGCT will be included. The search will be performed in May 2024 in the following databases: MEDLINE (Ovid), Embase (Ovid), Web of Science Core Collection and Google Scholar (100-top ranked).
Discussion:
This scoping review will identify knowledge and gaps in the current understanding of the molecular landscape of aGCT, clinical trials on actionable variations and priorities for future research. As aGCT are rare, a possible limitation will be the small sample sizes and heterogenic study settings.
Scoping Review Registration:
The review protocol is registered at Open Science Framework under https://doi.org/10.17605/OSF.IO/PX4MF.
Insights
This review maps DNA alterations and biomarkers in adult granulosa cell tumors (aGCT). It identifies knowledge gaps for rare tumor research and personalized treatment strategies.
Area of Science:
- Oncology
- Genomics
- Molecular Biology
Background:
- High-throughput sequencing advances tumor molecular profiling.
- Rare tumors like adult granulosa cell tumors (aGCT) present challenges in DNA sequencing due to small sample sizes.
- Understanding aGCT molecular landscape is crucial for personalized treatment and prognostic biomarker development.
Purpose of the Study:
- To systematically map existing evidence on DNA alterations in aGCT.
- To identify actionable variations and prognostic biomarkers for aGCT.
- To pinpoint knowledge gaps and guide future research in rare gynecologic cancers.
Main Methods:
- Scoping review following Arksey and O'Malley's framework and JBI Evidence Synthesis modifications.
- Adherence to PRISMA extension guidelines for scoping reviews.
- Comprehensive literature search across MEDLINE, Embase, Web of Science, and Google Scholar in May 2024.
Main Results:
- The review will synthesize findings on the molecular landscape of aGCT.
- It will identify current clinical trials investigating actionable variations.
- Knowledge gaps regarding genetic alterations and prognostic markers will be highlighted.
Conclusions:
- This scoping review will elucidate the current understanding of aGCT molecular characteristics.
- It will identify research priorities for actionable variations and prognostic biomarkers.
- Limitations include potential small sample sizes and study heterogeneity inherent in rare tumor research.
