Diterpenoid alkaloids from Delphinium trichophorum

Area of Science:

• Natural Product Chemistry
• Pharmacology
• Medicinal Chemistry

Background:

• Delphinium trichophorum is a plant species with potential medicinal properties.
• Diterpenoid alkaloids are a class of natural products known for diverse biological activities.

Purpose of the Study:

• To isolate and characterize novel diterpenoid alkaloids from Delphinium trichophorum.
• To evaluate the biological activities of the isolated compounds, including anti-inflammatory and acetylcholinesterase inhibition.

Main Methods:

• Phytochemical investigation of the ethanol extract of Delphinium trichophorum.
• Structure elucidation using extensive spectroscopic analyses (HSQC, HMBC, 1H-1H COSY, NOESY) and X-ray crystallography.
• Screening for inhibitory activities against LPS-induced NO production and acetylcholinesterase.

Main Results:

• Isolation and characterization of ten unprecedented diterpenoid alkaloids: nine delnudine-type (trichophodines A-I) and one kusnezoline-type (trichophozine A).
• Identification of seven known compounds.
• Guan-fu base V demonstrated potent acetylcholinesterase inhibitory activity (53.81% inhibition at 40 μM).

Conclusions:

• The study successfully identified novel diterpenoid alkaloids from Delphinium trichophorum.
• Guan-fu base V exhibits significant potential as an acetylcholinesterase inhibitor, warranting further investigation for therapeutic development.