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Complete mitogenome and intra-family comparative mitogenomics showed distinct position of Pama Croaker Otolithoides pama

  • 0Genetics and Molecular Biology Laboratory, Department of Zoology, University of Dhaka, Dhaka, 1000, Bangladesh.
Scientific Reports +

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June 15, 2024

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June 15, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

This study sequenced the Pama Croaker mitochondrial genome, aiding in distinguishing it from similar species. This research supports the conservation of this economically important fish and its genetic diversity.

Area Of Science

  • Marine Biology
  • Genomics
  • Ichthyology

Background

  • The Pama Croaker (Otolithoides pama) is vital to Bangladesh's fisheries.
  • Morphological similarities and barcode gene variations complicate accurate species identification.
  • Mitochondrial genome analysis offers a robust method for fish identification and phylogenetic studies.

Purpose Of The Study

  • To sequence and analyze the complete mitochondrial genome of Otolithoides pama.
  • To conduct comparative mitogenomics within the Sciaenidae family.
  • To improve the identification of O. pama and related species, aiding conservation efforts.

Main Methods

  • DNA extraction and Illumina-based short-read sequencing for de novo mitogenome assembly.
  • Analysis of mitogenome size, base composition, strand asymmetry, and codon usage.
  • Comparative analysis of mitogenomes from 30 Sciaenidae species, including phylogenetic tree construction.

Main Results

  • The complete mitogenome of O. pama (16,513 bp) was assembled, showing significant A+C bias and strand asymmetry.
  • Mitogenomes within Sciaenidae were generally consistent in size and gene order, with exceptions in the genus Johnius.
  • The 12S rRNA gene and control region were identified as conserved and variable regions, respectively; protein-coding genes were under purifying selection.

Conclusions

  • The study provides a high-quality mitogenome sequence for O. pama, crucial for species identification.
  • Phylogenetic analysis confirms the distinct placement of O. pama within the Sciaenidae family.
  • This research contributes to the genetic understanding and conservation of O. pama and related species.

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