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Updated: Jun 23, 2025
Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling
Published on: May 29, 2011
A ratiometric fluorescent probe based on a novel fluorophore with high selectivity for imaging cysteine in living
Zhuo Miao1, Cheng Li1, Shude Pang1
1Departments of Pharmaceutical Analysis and Pharmacology, School of Pharmacy, Qingdao University Medical College, Qingdao, China.
Abstract:
As a biothiol, cysteine (Cys) is essential to both physiological and pathological processes and has been associated with many diseases, including neurological disorders, rheumatoid arthritis, and renal dysfunction. Therefore, the development of a high-performance probe for detecting Cys levels can help prevent and diagnose disease. In this study, a ratiometric fluorescent probe based on a novel fluorophore was developed for detecting Cys, and it showed high specificity and a rapid response time toward Cys. This probe demonstrates excellent biocompatibility and has been utilized effectively for the imaging of Cys in living cells.
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