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Guanine-Rich RNA Sequence Binding Factor 1 Deficiency Promotes Colorectal Cancer Progression by Regulating PI3K/AKT

Jingzhan Huang1, Jialong Liu1, Jin Lan1

  • 1Department of General Surgery, The Third Affiliated Hospital, Southern Medical University, Guangzhou, Guangdong, People's Republic of China.

Cancer Management and Research
|June 17, 2024
PubMed
Abstract

Insights

Guanine-rich RNA sequence binding factor 1 (GRSF1) is downregulated in colorectal cancer (CRC), promoting tumor growth. Restoring GRSF1 may offer a new therapeutic strategy for CRC patients.

Area of Science:

  • Molecular biology
  • Oncology
  • Cancer research

Background:

  • Guanine-rich RNA sequence binding factor 1 (GRSF1) is an RNA-binding protein implicated in various human cancers.
  • The specific role and molecular mechanisms of GRSF1 in colorectal cancer (CRC) progression remain unclear.

Purpose of the Study:

  • To investigate the expression and function of GRSF1 in colorectal cancer.
  • To determine the molecular mechanisms underlying GRSF1's role in CRC.
  • To evaluate GRSF1 as a potential therapeutic target and biomarker for CRC.

Main Methods:

  • Immunohistochemistry and Western Blot to assess GRSF1 mRNA and protein levels in CRC.
  • Functional assays to evaluate GRSF1's impact on CRC cell proliferation, migration, and invasion.
  • Analysis of the PI3K/Akt signaling pathway and Ras activation in relation to GRSF1 expression.

Main Results:

  • GRSF1 expression is universally downregulated in CRC, correlating with poor prognosis.
  • Loss of GRSF1 enhances CRC cell proliferation, migration, and invasion.
  • Overexpression of GRSF1 inhibits these tumor-promoting activities.

Conclusions:

  • GRSF1 functions as a tumor suppressor gene in colorectal cancer.
  • GRSF1 insufficiency modulates the PI3K/Akt signaling pathway and Ras activation.
  • GRSF1 presents a promising biomarker and potential therapeutic target for CRC management.
Keywords:
CRCGRSF1Ras/PI3K/Aktmetastasisproliferation

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