Related Experiment Video

Updated: Jun 23, 2026

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

19.7K

The Microbiome Protocols eBook initiative: Building a bridge to microbiome research

Yunyun Gao1, Kai Peng2, Defeng Bai1

  • 1Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural Sciences Shenzhen China.

Imeta
|June 17, 2024
PubMed

Insights

The Microbiome Protocols eBook (MPB) bridges gaps in experimental and data analysis protocols. We invite researchers to contribute to the second edition, advancing microbiome research globally.

Area of Science:

  • Microbiology
  • Bioinformatics
  • Molecular Biology

Background:

  • The Microbiome Protocols eBook (MPB) was established to address critical gaps in standardized methodologies for microbiome research.
  • The first edition, released in 2020, successfully compiled 152 protocols, receiving positive feedback from the scientific community.

Purpose of the Study:

  • To announce and invite contributions for the second edition of the Microbiome Protocols eBook (MPB).
  • To expand the collection of validated protocols for both wet-lab experiments and computational analysis in microbiome science.

Main Methods:

  • This initiative involves a community-driven approach, soliciting novel and established protocols from researchers worldwide.
  • Submitted protocols will undergo a curation process to ensure quality and relevance.

Main Results:

  • The first edition of the MPB provided a valuable resource, demonstrating a clear need for such a compilation.
  • The ongoing development of the second edition aims to further enhance the accessibility and comprehensiveness of microbiome research methods.

Conclusions:

  • The Microbiome Protocols eBook (MPB) is an essential, evolving resource for the microbiome research community.
  • Continued contributions are vital for maintaining the MPB as a leading reference for experimental and analytical protocols.

More Related Videos

Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments10:31

Isolation and Analysis of Microbial Communities in Soil, Rhizosphere, and Roots in Perennial Grass Experiments

Published on: July 24, 2018

54.7K
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing11:22

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

27.9K

Related Concept Videos

Bacterial Signaling01:30

Bacterial Signaling

Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Synthetic Biology02:55

Synthetic Biology

Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
Microorganisms in Medicine and Therapeutics01:29

Microorganisms in Medicine and Therapeutics

Microorganisms play a fundamental role in vaccine development, gene therapy, and therapeutic production. Their biological properties are harnessed to advance medicine and public health. Beyond immunization, microorganisms contribute to gut health, antibiotic synthesis, and genetic disease treatment.Live Attenuated and Inactivated VaccinesLive attenuated vaccines, such as the measles, mumps, and rubella (MMR) vaccine, utilize weakened forms of pathogens to closely resemble natural infections.
Microbial Interactions: Cooperation01:26

Microbial Interactions: Cooperation

Microbial cooperation involves beneficial interactions in which different species work together for individual or mutual advantage. These interactions can profoundly influence ecological dynamics and evolutionary processes, and they are essential to many pathogenic and symbiotic relationships.Nematode–Bacteria CooperationA striking example is the relationship between the Gram-negative bacterium Xenorhabdus nematophila and the parasitic nematode Steinernema carpocapsae. Juvenile nematodes...
Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...