Related Experiment Video
Updated: Jun 23, 2025
Author Spotlight: Genetic Profiling for Fluorouracil Response in Gastric Cancer
Published on: May 10, 2024
Effect of MTHFR A1298C Gene Polymorphism on Acute Coronary Syndrome
Muhamad Robiul Fuadi1, Jusak R Nugraha2, I Gde Rurus Suryawan3
1Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
Insights
The methylenetetrahydrofolate reductase (MTHFR) A1298C gene variant does not significantly impact acute coronary syndrome (ACS) incidence. This genetic factor was not found to be a risk factor for ACS in the studied Indonesian population.
Area of Science:
- Genetics and Cardiovascular Health
- Molecular Biology and Disease Mechanisms
- Population Health and Epidemiology
Background:
- Cardiovascular disease (CVD) is a leading global cause of death.
- Acute coronary syndrome (ACS) is a critical manifestation of CVD.
- Genetic predisposition to ACS requires further investigation, particularly in Indonesia.
Purpose of the Study:
- To investigate the association between methylenetetrahydrofolate reductase (MTHFR) A1298C gene polymorphism and ACS incidence.
- To evaluate MTHFR A1298C as a potential genetic risk factor for ACS in an Indonesian cohort.
Main Methods:
- Case-control study design involving ACS patients and healthy controls.
- Genotyping of MTHFR A1298C polymorphism using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
- Statistical analysis including Chi-square and odds ratio calculation to determine association.
Main Results:
- Genotype frequencies (AA, AC, CC) were analyzed in both ACS and control groups.
- The C allele was present in 41% of the ACS group and 37% of the control group.
- No statistically significant association was found between MTHFR A1298C polymorphism and ACS incidence (P > 0.05).
Conclusions:
- The MTHFR A1298C gene polymorphism is not a significant determinant of ACS incidence.
- This genetic variant does not appear to confer increased risk for acute coronary syndrome in the studied population.
Background:
Cardiovascular disease (CVD) is the leading cause of mortality worldwide. Acute coronary syndrome is a manifestation of CVD. In Indonesia, limited studies have been conducted on genetics as a potential risk factor for acute coronary syndrome (ACS). Consequently, this study aimed to examine the effect of the methylenetetrahydrofolate reductase (MTHFR) A1298C gene polymorphism on the incidence of ACS.
Method:
The study employed a case-control design. Outpatients from the cardiology and internal medicine clinics at the University of Airlangga (UNAIR) Hospital in Surabaya, Indonesia, constituted the study population. The case group comprised 60 patients with a history of ACS, while the control group consisted of 30 patients without a history of cardiovascular complaints. MTHFR A12980C gene polymorphism examination was performed using the polymerase chain reaction-restriction fragment length polymorphism (PCR RFLP) method at the Tropical Disease Center UNAIR Laboratory.
Results:
Among the ACS group, 29 (48.1%), 13 (21.7%), and 18 (30%) of the individuals had AA, AC, and CC genotype patterns, respectively. In the control group, 16 individuals had AA (53.3%), 6 AC (20%), and 8 CC (26.7%). The C allele variant was identified in 41% of the ACS group and 37% of the control group. The odds ratio (OR) for the incidence of ACS was 1.195 (95% confidence interval [CI]; 0.381-3.752), 1.241 (95% CI; 0.481-3.486), and 1.222 (95% CI; 0.381-3.752). Chi-square analysis revealed no association between MTHFR A1298C gene polymorphism and the incidence of ACS (P > 0.05).
Conclusions:
MTHFR A1298C gene polymorphism did not significantly affect ACS incidence.
More Related Videos
07:00A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene
Published on: April 1, 2019
07:26High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment
Published on: July 18, 2017
