Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Advancing Italian biophysics: insights from the 27th SIBPA congress.

European biophysics journal : EBJ·2026

Same author

Antisecretory Factor in rat cerebellar granule cells augments inhibitory current via GABA<sub>A</sub> receptors: a mechanism for vertigo reduction in Menière's disease?

European biophysics journal : EBJ·2026

Same author

Wavefront estimation through structured detection in laser scanning microscopy.

Biomedical optics express·2026

Same author

Correction of Background in Fluorescence Correlation Spectroscopy for Accurate Determination of Particle Number.

Biomolecules·2026

Same author

Darkfield Mueller matrix microscope allows the observation of single cells with high contrast.

Optics letters·2025

Same author

Light-enhanced cytotoxicity and intracellular trafficking of the PD-L1-targeting photoimmunoconjugate EITC-atezolizumab.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2025

Same journal

Application of Resonant Plasmonic Bowtie Nanoantennas for Optically-Assisted Diffusiophoretic Trapping of Extracellular Vesicles and Nanoparticles.

Laser & photonics reviews·2026

Same journal

Ultranarrow linewidth photonic-atomic laser.

Laser & photonics reviews·2026

Same journal

Free-Space-Coupled Frequency-Locked Microtoroid Resonators with Reactive Polymer Functionalization for Part-Per-Trillion Gas Detection.

Laser & photonics reviews·2026

Same journal

On-Chip Multimode WGM Microresonator with Cross-Correlation Algorithm for Enhanced Sensing.

Laser & photonics reviews·2025

Same journal

Cavity Optomechanical Bistability with an Ultrahigh Reflectivity Photonic Crystal Membrane.

Laser & photonics reviews·2025

Same journal

Pixelation with concentration-encoded effective photons for quantitative molecular optical sectioning microscopy.

Laser & photonics reviews·2025

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 23, 2025

Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling

Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling

Published on: October 11, 2022

Roadmap on Label-Free Super-Resolution Imaging.

Vasily N Astratov¹, Yair Ben Sahel², Yonina C Eldar²

¹Department of Physics and Optical Science, University of North Carolina at Charlotte, Charlotte, North Carolina 28223-0001, USA.

Laser & Photonics Reviews

|June 17, 2024

Summary

This summary is machine-generated.

Label-free super-resolution (LFSR) imaging bypasses fluorescent staining by using light scattering for nanoscale visualization. This roadmap outlines current advancements and future directions to surpass the diffraction limit in LFSR imaging.

Keywords:

Raman microscopy absorption artificial intelligence biomedical imaging confocal microscopy deep learning diffraction diffraction limit focusing high-resolution imaging holography interference label-free imaging metamaterials microlens design microresonators microspheres nanoplasmonics near-field imaging optical microscopy photonic crystals polarization propagation reflectivity scattering solid immersion lens spectroscopy structured illumination super-resolution superlens tomography transformation optics

More Related Videos

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Published on: November 5, 2017

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Related Experiment Videos

Last Updated: Jun 23, 2025

Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling

Label-Retention Expansion Microscopy LR-ExM Enables Super-Resolution Imaging and High-Efficiency Labeling

Published on: October 11, 2022

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Published on: November 5, 2017

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Area of Science:

Optics and Photonics
Biomedical Imaging
Nanotechnology

Background:

Label-free super-resolution (LFSR) imaging utilizes light scattering, avoiding fluorescent staining needed in conventional super-resolved microscopy.
Current LFSR techniques offer high axial and temporal resolution but face challenges in achieving true lateral super-resolution.

Purpose of the Study:

To present a comprehensive vision of LFSR imaging developments and the current state-of-the-art.
To identify resolution boundaries and challenges in LFSR imaging, aiming to overcome the classical diffraction limit.
To foster collaboration between physics and biomedical optics researchers in LFSR imaging.

Main Methods:

Advanced interference detection techniques for enhanced axial and temporal resolution.
Structured illumination, near-field scanning, and nonlinear optics for lateral super-resolution.
Superlenses utilizing nanoplasmonics, metamaterials, transformation optics, and microsphere-assisted approaches.

Main Results:

Exploration of diverse physical mechanisms for LFSR imaging.
Identification of key areas for future research and development in LFSR.
Bridging the gap between theoretical physics and practical biomedical applications.

Conclusions:

LFSR imaging holds significant potential for surpassing the diffraction limit.
Interdisciplinary collaboration is crucial for advancing LFSR technology.
This roadmap sets the stage for future research in label-free super-resolution imaging.