Related Experiment Video
Updated: Jul 18, 2026

Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes
Published on: November 9, 2020
mRNA splicing efficiency in yeast and the contribution of nonconserved sequences
Abstract:
A simple kinetic model for mRNA splicing predicts the way in which in vivo steady state precursor RNA levels (P) and messenger RNA levels (M) vary as a function of the rate constant of the splicing reaction (ksp). The model points to M/P as the best measure of ksp. The analysis of a set of intron mutations in a yeast gene supports the general features of the model and shows that the splicing efficiency of transcripts containing the wild-type intron is well in excess of what is necessary to generate normal mRNA levels. The data also suggest that regions of the intron, in addition to the well-conserved consensus sequences, contribute to efficient splicing.
Related Concept Videos
RNA Splicing
RNA Splicing
Nonsense-mediated mRNA Decay
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Alternative RNA Splicing
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Pre-mRNA Processing: RNA Splicing
Alternative RNA Splicing
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...

