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Updated: May 12, 2026
13:50
Sequencing of Bacterial Microflora in Peripheral Blood: our Experience with HIV-infected Patients
Published on: June 11, 2011
12.3K
Deciphering HIV-associated inflammation: microbiome's influence and experimental insights.
Ricky A Lippincott1, John O'Connor2, Charles P Neff1
1Department Medicine.
Current Opinion in HIV and AIDS
|June 17, 2024
Summary
Novel experimental approaches are reviewed to study host:microbe interactions and inflammation in people living with HIV (PLWH). Multiomic data guides investigations, while in-vitro and in-vivo assays identify causal factors.
Area of Science:
- Microbiology
- Immunology
- Gastroenterology
Background:
- Inflammation in people living with HIV (PLWH) is influenced by complex host:microbe interactions within the gut.
- Understanding these interactions is crucial for managing intestinal and systemic inflammation in PLWH.
- Current knowledge gaps necessitate advanced research methodologies.
Purpose of the Study:
- To review innovative experimental techniques for investigating host:microbe interactions.
- To highlight the role of these interactions in intestinal and systemic inflammation in PLWH.
- To provide an overview of current research strategies in this field.
Main Methods:
- Multiomic systems biology approaches for generating hypothesis-driven data.
- In-vitro models including peripheral blood mononuclear cells (PBMCs), lamina propria mononuclear cells (LPMCs), and human intestinal organoids (HIOs).
- In-vivo models such as nonhuman primates and germ-free/humanized mice for studying host-microbiome relationships.
Main Results:
- Multiomic data offers valuable insights into host:microbe interactions, guiding further research.
- In-vitro and in-vivo models allow for the elucidation of direct host-cell-microbe interactions and broader host-microbiome dynamics.
- These approaches are essential for dissecting the complex inflammatory landscape in PLWH.
Conclusions:
- Multiomic data generation is key for formulating hypotheses in host:microbe research.
- In-vitro and in-vivo assays are critical for identifying causal factors in host-microbe interactions.
- Integrated experimental strategies are vital for advancing our understanding of inflammation in PLWH.
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