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Related Experiment Video

Updated: Jun 23, 2025

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Generation of Genetically Modified Mice through the Microinjection of Oocytes

Published on: June 15, 2017

Techniques for Genetic Manipulation.

Carla García López^1,2, Luis Saralegui Remón^1,2, Cristina Uruén García^1,2

¹Unidad de Microbiología e Inmunología, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain.

Methods in Molecular Biology (Clifton, N.J.)

|June 17, 2024

Summary

This study details methods for genetic modification in Streptococcus suis (S. suis), focusing on DNA construct preparation and uptake techniques. These protocols are essential for advancing genotype and phenotype research in S. suis.

Keywords:

Genetic engineering Mutants Plasmid Streptococcus suis TransformationTransformation

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Last Updated: Jun 23, 2025

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Published on: May 23, 2016

Area of Science:

Molecular Biology
Genetics
Microbiology

Background:

Streptococcus suis (S. suis) is a significant pathogen.
Molecular and genetic techniques are crucial for studying S. suis genotypes and phenotypes.
Genomic modification of S. suis necessitates efficient DNA acquisition and chromosomal integration via allelic exchange.

Purpose of the Study:

To describe established protocols for genomic modification of S. suis.
To provide detailed methods for preparing genomic constructs and facilitating DNA uptake.
To enable researchers to perform genetic studies on S. suis.

Main Methods:

Preparation of genomic constructs using cloning and overlapping extension PCR.
DNA uptake into S. suis via electroporation and transformation.
Application of protocols to the reference S. suis strain P1/7.

Main Results:

Successful implementation of described protocols for genomic construct preparation.
Demonstrated efficacy of electroporation and transformation for DNA uptake in S. suis.
Validation of the protocols using the reference S. suis strain P1/7.

Conclusions:

The described molecular techniques provide reliable methods for S. suis genomic modification.
These protocols are valuable tools for advancing research into S. suis pathogenesis and genetics.
Successful application ensures researchers can effectively manipulate S. suis genome.