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A deep learning method to predict bacterial ADP-ribosyltransferase toxins.
Dandan Zheng1, Siyu Zhou1, Lihong Chen1
1NHC Key Laboratory of Systems Biology of Pathogens, National Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 102629, China.
Bioinformatics (Oxford, England)
|June 17, 2024
Summary
A new deep learning model, ARTNet, accurately identifies bacterial ADP-ribosyltransferase toxins (bARTTs) in genomes. This tool aids in discovering novel bARTTs, crucial for understanding bacterial virulence and pathogenesis.
Area of Science:
- Biochemistry and Molecular Biology
- Bioinformatics and Computational Biology
- Microbiology
Background:
- ADP-ribosylation regulates key cellular processes like chromatin structure, RNA transcription, and cell death.
- Bacterial ADP-ribosyltransferase toxins (bARTTs) are vital virulence factors enabling bacteria to manipulate host cells for pathogenesis.
- Identifying novel bARTTs is challenging due to limited data and sequence diversity.
Purpose of the Study:
- To develop a deep learning model, ARTNet, for accurate prediction of bARTTs from bacterial genomes.
- To overcome data scarcity and enhance prediction performance through data augmentation and domain subsequence utilization.
Main Methods:
- Developed ARTNet, a deep learning model for bARTT prediction.
- Implemented data augmentation techniques to address limited training data.
- Utilized ART-related domain subsequences for optimized model performance.
Main Results:
- ARTNet achieved high performance with an MCC of 0.9351 and F1-score (macro) of 0.9666 on independent test datasets.
- ARTNet outperformed existing deep learning and traditional machine learning models in accuracy and efficiency.
- Demonstrated ARTNet's capability to predict novel bARTTs across diverse superfamilies, even without sequence similarity.
Conclusions:
- ARTNet is an effective tool for identifying novel bacterial ADP-ribosyltransferase toxins.
- The model significantly advances the screening and discovery of bARTTs in bacterial genomics.
- ARTNet is publicly available, facilitating broader research in bacterial pathogenesis and virulence.
