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Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
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Rft1 catalyzes lipid-linked oligosaccharide translocation across the ER membrane

Shuai Chen1,2,3, Cai-Xia Pei4,5, Si Xu1

  • 1Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, China.

Nature Communications
|June 17, 2024
PubMed
Summary

Researchers confirm Rft1 as the enzyme responsible for flipping Man5GlcNAc2-PP-Dol (M5GN2-PP-Dol) across the endoplasmic reticulum membrane. This resolves a long-standing controversy regarding the M5GN2-PP-Dol ER flippase in protein glycosylation.

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