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Integrated bioinformatics analysis and experimental validation identifies CPE as a potential biomarker and

Xiaozhen Peng1,2,3, Yun Zhong1,3, Rui Mao1,3

  • 1Hunan key laboratory of aging biology, Xiangya Hospital, Central South University, Changsha, China.

Experimental Dermatology
|June 18, 2024
PubMed

Insights

Carboxypeptidase E (CPE) is identified as a key marker for skin aging. Lowering CPE accelerates skin aging, while increasing it delays aging, suggesting CPE as a therapeutic target.

Area of Science:

  • Molecular Biology
  • Dermatology
  • Gerontology

Background:

  • Aging is a natural process involving physiological decline, with limited predictive biomarkers and therapies.
  • Understanding skin aging mechanisms is crucial for developing effective interventions.

Purpose of the Study:

  • To identify pivotal biomarkers for skin aging using advanced computational techniques.
  • To investigate the role of Carboxypeptidase E (CPE) in the aging process of human dermal fibroblasts.

Main Methods:

  • Weighted Gene Co-expression Network Analysis (WGCNA) and machine learning on transcriptome data.
  • Analysis of CPE expression in various induced senescence models of human dermal fibroblasts (HDFs).
  • Experimental manipulation of CPE levels (knockdown and overexpression) in HDFs.

Main Results:

  • Carboxypeptidase E (CPE) was identified as a pivotal marker of skin aging.
  • CPE expression is downregulated in senescent HDFs (replicative, UVA, and H2O2-induced).
  • CPE knockdown accelerates HDF senescence; CPE overexpression delays it, inhibiting collagen loss and inflammation.

Conclusions:

  • CPE acts as a significant predictor of skin aging.
  • CPE demonstrates potential as a therapeutic target for mitigating skin aging.
  • Modulating CPE levels offers a novel strategy for skin aging intervention.
Keywords:
CPEWGCNAageingmachine learningscRNA

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