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[ELISA detection of protamine antibodies]
Immunitat Und Infektion
|April 1, 1985
Abstract:
Protamine is frequently used as an adjuvant in insulin preparations. As alien protein protamine is immunogenic. We developed an ELISA for detection of protamine antibodies. As a strongly basic molecule protamine shows remarkable reactivity. Resulting methodical difficulties with regard to the assay are discussed. The course of antibody titres to protamine after immunization is shown in rabbits, goats, sheep and guinea pigs with positive results in all species. In humans protamine antibodies were detectable in 4% of 150 patients treated with protamine insulins.