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Related Experiment Video

Updated: Jun 23, 2025

Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro

Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro

Published on: January 25, 2017

A high-dimensional platform for observing neutrophil-parasite interactions.

Brandon A Thompson¹, Julio Revilla¹, Savannah Brovero¹

¹Division of Infectious Diseases and International Health, Department of Internal Medicine, University of Virginia School of Medicine, Charlottesville, Virginia, USA.

Microbiology Spectrum

|June 18, 2024

Summary

Neutrophils combat Entamoeba histolytica infection, with Clostridium scindens enhancing this protective immune response. This study introduces a novel spectral flow cytometry assay to analyze these critical host-parasite interactions.

Keywords:

Entamoeba amebiasis gut microbiome neutrophils parasitology spectral flow

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Last Updated: Jun 23, 2025

Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro

Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro

Published on: January 25, 2017

Bioparticle Microarrays for Chemotactic and Molecular Analysis of Human Neutrophil Swarming in vitro

Bioparticle Microarrays for Chemotactic and Molecular Analysis of Human Neutrophil Swarming in vitro

Published on: February 16, 2020

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

Published on: June 3, 2014

Area of Science:

Immunology
Infectious Diseases
Microbiology

Background:

Diarrheal diseases caused by Entamoeba histolytica are a significant global health burden, particularly in low-income nations.
Neutrophils play a protective role in E. histolytica infections, but the mechanisms of parasite killing are not fully understood.
Existing tools for studying host immune cell-E. histolytica interactions are limited, hindering research progress.

Purpose of the Study:

To develop and validate a novel assay for analyzing neutrophil-E. histolytica interactions using high-dimensional spectral flow cytometry.
To investigate the impact of Clostridium scindens colonization on neutrophil-mediated killing of E. histolytica.
To establish a platform for in-depth analysis of granulocyte-parasite interactions in various host conditions.

Main Methods:

Isolation of neutrophils from wild-type C57BL/6 mice.
Co-culture of neutrophils with E. histolytica at various multiplicities of infection (MOIs).
Staining and analysis of co-cultured cells using high-dimensional spectral flow cytometry with specific surface markers and FMO controls.

Main Results:

Development of a novel spectral flow cytometry assay to quantify granulocyte-parasite interactions.
Demonstration that neutrophils from mice colonized with Clostridium scindens exhibit enhanced killing capacity against E. histolytica compared to controls.
Confirmation that C. scindens colonization is associated with elevated neutrophil counts and improved amebic killing.

Conclusions:

The developed spectral flow cytometry platform provides a powerful tool for detailed analysis of host-parasite interactions.
Alterations in the intestinal microbiota, such as C. scindens colonization, can modulate neutrophil function and enhance E. histolytica killing.
This platform facilitates precise hypothesis testing regarding host-parasite dynamics in infectious diseases.