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Related Concept Videos

Tooth Anatomy01:21

Tooth Anatomy

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The human tooth enables us to eat a variety of foods, speak clearly, and even aid in shaping our faces. Teeth are composed of various elements that work together. Here's a detailed look at the anatomy of a human tooth.
The Crown, Neck, and Root
The visible part of the tooth is referred to as the crown. It's covered by enamel, the hardest substance in the human body. The crown is uniquely shaped for each type of tooth, allowing for different functions such as cutting, tearing, or...
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Isolation, Processing and Analysis of Murine Gingival Cells
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Development of 3D gingival in vitro models using primary gingival cells.

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  • 1Ashland Specialties France, 655 Route du Pin Montard, 06904, Sophia Antipolis, France. cplaza@ashland.com.

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|June 18, 2024
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European cosmetic regulations ban animal testing. Researchers developed an in-house 3D gingival model using human cells to assess ingredient safety and test oral care formulations.

Keywords:
3D in vitro modelsConjunctival equivalentGingival epithelium

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Area of Science:

  • Biomedical Engineering
  • Tissue Engineering
  • Cosmetic Science

Background:

  • European Union banned animal testing for cosmetics in March 2013.
  • This ban includes oral care ingredients, necessitating alternative safety assessment methods.
  • Existing in vitro gingival models lack customization for specific research needs.

Purpose of the Study:

  • To develop an in-house, customizable 3D human gingival model.
  • To enable modulation of parameters for studying oral diseases and ingredient toxicity.
  • To evaluate biocompatibility and formulations of cosmetic ingredients.

Main Methods:

  • Utilized tissue engineering expertise to reconstruct human oral tissues.
  • Isolated and cultured primary human gingival fibroblasts and keratinocytes.
  • Constructed gingival epithelium, chorion equivalents, and full-thickness models.

Main Results:

  • Cultured gingival cells retained proliferative capacity, enabling tissue reconstruction.
  • Reconstructed gingival tissues exhibited characteristics similar to in vivo human gingival models.
  • The developed model allows for modulation of various parameters for research.

Conclusions:

  • The in-house 3D gingival model provides a viable alternative to animal testing for cosmetic ingredients.
  • This model supports comprehensive safety and efficacy evaluations for oral care products.
  • The technology facilitates personalized research into oral diseases and ingredient interactions.