Cutting-edge HPLC and MCR techniques for synchronically quantifying anticholinergic drugs in the presence of C12 and C14 homologs: Robust application to green and white chemistry
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
View abstract on PubMed
Summary
This summary is machine-generated.This study introduces nine green and white chemistry metrics to assess the environmental impact of analytical methods for cyclopentolate and benzalkonium chloride. The developed methods demonstrate high accuracy and recovery rates, promoting sustainable chemical analysis.
Area Of Science
- Green Chemistry
- Analytical Chemistry
- Pharmaceutical Analysis
Background
- Green and white chemistry are crucial for enhancing sustainability and efficiency in the chemical industry.
- Developing robust analytical methods is essential for quality control in pharmaceutical formulations.
- Assessing the environmental footprint of analytical procedures is increasingly important.
Purpose Of The Study
- To develop and apply nine sustainability tools (green and white metrics) for evaluating the environmental friendliness of analytical methods.
- To concurrently analyze and separate cyclopentolate (CYC) and benzalkonium chloride (BNZ) homologs.
- To compare the performance of mean centering of ratio spectra (MCR) and reversed-phase high-performance liquid chromatography (RP-HPLC) strategies.
Main Methods
- Utilized nine sustainability metrics: GAPI, ComplexGAPI, analytical greenness, analytical greenness metric for sample preparation, ESA, analytical method greenness score, HPLC-EAT, analytical method volume intensity, and BAGI.
- Employed mean centering of ratio spectra (MCR) for spectral overlapping resolution and RP-HPLC for separation.
- Optimized mobile phase (phosphate buffer:acetonitrile, 35:65, v/v) at pH 6.3, UV detection at 215 nm, flow rate 2.0 mL/min, and column L11 Inertsil Ph-3 at 25°C.
Main Results
- MCR effectively resolved spectral overlapping for CYC (227.5 nm) and BNZ (220.4 nm).
- RP-HPLC employed an isocratic method with high linearity (correlation coefficient > 0.999) for both methods.
- Excellent recovery rates (97.2%–100.5%) were achieved, and the methods were validated per ICH guidelines.
Conclusions
- The developed analytical strategies, assessed by multiple green and white metrics, are environmentally friendly and efficient.
- The MCR and RP-HPLC methods provide accurate and reliable quantification of CYC and BNZ in pure and ophthalmic solutions.
- This study demonstrates the successful application of sustainability tools in evaluating and optimizing analytical methods for pharmaceutical analysis.
These videos have been matched automatically. Contact us if they are not relevant.
These videos have been matched automatically. Contact us if they are not relevant.
Related Concept Videos
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
Mobile Phase: This is a liquid solvent that flows through the system, carrying the sample with...
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...