Detection of synergistic hemolytic activity of Staphylococcus aureus with the Cathra replicator

A replicator method for screening Staphylococcus aureus isolates for synergistic hemolytic activity is described. The technique should also be useful for assessing the hemolytic activity of staphylococci and streptococci on different lots or brands of blood agar because the inoculum and the distances between spot inocula are standardized.