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Updated: Jun 23, 2025

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Circ6834 suppresses non-small cell lung cancer progression by destabilizing ANHAK and regulating miR-873-5p/TXNIP

Maoye Wang1, Xiaoge Ding1, Xinjian Fang2

  • 1Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, 212013, China.

Molecular Cancer
|June 18, 2024
PubMed
Abstract

Insights

A novel circular RNA, circ6834, is downregulated in non-small cell lung cancer (NSCLC). Overexpression of circ6834 suppresses NSCLC progression by inhibiting the TGF-β/Smad signaling pathway, offering a new therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are implicated in cancer progression and metastasis.
  • The specific roles of circRNAs in non-small cell lung cancer (NSCLC) are not fully understood.

Purpose of the Study:

  • To identify and characterize novel circRNAs in NSCLC.
  • To investigate the biological functions and molecular mechanisms of circ6834 in NSCLC progression.

Main Methods:

  • RNA sequencing (RNA-seq) to identify circRNAs.
  • In vitro and in vivo experiments to assess circ6834 function.
  • Tagged RNA affinity purification (TRAP), western blot, RNA immunoprecipitation, dual luciferase reporter assays, and rescue experiments to elucidate molecular mechanisms.

Main Results:

  • hsa_circ_0006834 (circ6834) was identified and found to be downregulated in NSCLC tissues and cell lines.
  • Circ6834 overexpression inhibited NSCLC cell growth and metastasis; knockdown promoted these processes.
  • Circ6834 antagonized TGF-β-induced epithelial-mesenchymal transition (EMT) and metastasis by interacting with AHNAK and sponging miR-873-5p, thereby inactivating the TGF-β/Smad signaling pathway.

Conclusions:

  • Circ6834 acts as a tumor suppressor in NSCLC.
  • Circ6834 inhibits NSCLC progression via a negative feedback loop with the TGF-β/Smad signaling pathway.
  • Circ6834 represents a potential therapeutic target for NSCLC.
Keywords:
CircRNANSCLCProgressionTGF-βTherapeutic targetcirc6834

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