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Instrumentation of Near-term Fetal Sheep for Multivariate Chronic Non-anesthetized Recordings

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Increasing GSH-Px Activity and Activating Wnt Pathway Promote Fine Wool Growth in FGF5-Edited Sheep.

Xue-Ling Xu^1,2, Su-Jun Wu¹, Shi-Yu Qi¹

¹Beijing Key Laboratory for Animal Genetic Improvement, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.

|June 19, 2024

Summary

Editing Fibroblast Growth Factor 5 (FGF5) in sheep increased fine wool density and improved wool quality. FGF5 gene editing also enhanced skin proliferation and regulated key hair regeneration pathways.

Keywords:

FGF5 Wnt antioxidant cortisol proliferation secondary hair follicle

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Area of Science:

Animal genetics
Dermatology
Molecular biology

Background:

Fibroblast Growth Factor 5 (FGF5) is crucial for hair follicle cycling, regulating the transition from anagen to catagen.
Sheep are valuable models for hair growth research and studies on human skin conditions.

Purpose of the Study:

To investigate the role of FGF5 in sheep hair growth using gene editing.
To assess the impact of FGF5 editing on wool quality, skin proliferation, and related molecular pathways.

Main Methods:

CRISPR/Cas9 gene editing was employed to create FGF5-edited Dorper sheep.
Analysis included measuring FGF5 levels, wool density and diameter, skin proliferation signals, cortisol concentration, antioxidant enzyme activity, and Wnt pathway gene expression.

Main Results:

FGF5 levels were significantly reduced in gene-edited (GE) sheep.
GE sheep exhibited increased fine wool density and a higher proportion of finer wool fibers (14.4-20.0 μm).
Skin proliferation signals were stronger in GE sheep, accompanied by decreased cortisol and activated antioxidant enzymes (GSH-Px).

Conclusions:

FGF5 editing positively influences secondary hair follicle (SHF) development in sheep.
FGF5 plays a role in mediating cortisol's activation of antioxidant pathways and influences Wnt signaling in hair regeneration.
FGF5 serves as a potential molecular marker for fine wool growth and can be targeted for improving wool quality in sheep breeding.