Related Experiment Video

Updated: Jun 23, 2025

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium
08:46

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Published on: September 1, 2015

9.8K

Increased Expression of Orexin-A in Patients Affected by Polycystic Kidney Disease

Ersilia Nigro1,2, Daniela D'Arco2, Fiorenzo Moscatelli3

  • 1Dipartimento di Scienze e Tecnologie Ambientali, Biologiche, Farmaceutiche, Università della Campania "Luigi Vanvitelli", Via Vivaldi 43, 81100 Caserta, Italy.

International Journal of Molecular Sciences
|June 19, 2024
PubMed

Insights

Elevated orexin-A levels are linked to impaired kidney function in autosomal dominant polycystic kidney disease (ADPKD) patients. This neuropeptide may play a role in renal and cardiovascular complications.

Area of Science:

  • Neuropeptide signaling
  • Renal physiology
  • Cardiovascular health

Background:

  • The orexinergic system, involving orexin-A, regulates feeding, sleep, and pressure.
  • Recent findings suggest a negative correlation between orexin-A levels and renal function.
  • Autosomal dominant polycystic kidney disease (ADPKD) is a genetic disorder affecting kidney function.

Purpose of the Study:

  • To investigate orexin-A levels and genetic variations in patients with ADPKD.
  • To assess the correlation between orexin-A levels and clinical features of ADPKD.
  • To explore the role of orexin-A in renal function and associated complications in ADPKD.

Main Methods:

  • Serum orexin-A levels were measured using ELISA.
  • Single nucleotide polymorphisms (SNPs) in HCRT, HCRTR1, and HCRTR2 were analyzed via Sanger sequencing.
  • Correlations between orexin-A, SNPs, and clinical parameters (e.g., eGFR, blood pressure) were assessed in 64 ADPKD patients and 24 healthy controls.

Main Results:

  • ADPKD patients exhibited impaired renal function (mean eGFR 67.8 ± 34.53) and higher systolic blood pressure compared to controls.
  • Serum orexin-A levels were significantly higher in ADPKD patients (477.07 ± 69.42 pg/mL) than in healthy controls (321.49 ± 78.01 pg/mL).
  • Orexin-A levels showed an inverse correlation with blood pressure and a direct correlation with estimated glomerular filtration rate (eGFR) in ADPKD patients. No significant SNPs were associated with orexin-A levels.

Conclusions:

  • Orexin-A plays a significant role in renal physiology and may be relevant to ADPKD.
  • Elevated orexin-A levels in ADPKD patients suggest its involvement in the disease's progression and associated cardiovascular issues.
  • Further research is needed to understand orexin-A signaling mechanisms in renal function and its therapeutic potential for ADPKD.
Keywords:
blood pressuremarkerorexin-Apolycystic kidney diseaserenal function

More Related Videos

Use of Ultra-high Field MRI in Small Rodent Models of Polycystic Kidney Disease for In Vivo Phenotyping and Drug Monitoring07:35

Use of Ultra-high Field MRI in Small Rodent Models of Polycystic Kidney Disease for In Vivo Phenotyping and Drug Monitoring

Published on: June 23, 2015

11.5K
Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice10:37

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

132

Related Concept Videos

Nephrons01:10

Nephrons

The kidneys are intricate organs with millions of working units known as nephrons. Each nephron features two major structures: the renal corpuscle, which facilitates blood plasma filtration, and the renal tubule, which handles the glomerular filtrate. Blood supply is directly linked to the nephrons. The renal corpuscle consists of the glomerulus, a capillary network, and the Bowman's capsule, a double-walled epithelial structure that encases the glomerulus. The filtering of blood plasma...
2.3K
Sleep-Wake Cycles01:24

Sleep-Wake Cycles

Sleep is an essential physiological process vital to maintaining overall well-being. The reticular activating system (RAS), a network of neurons in the brainstem, regulates wakefulness and sleep. While it may seem passive, sleep consists of distinct cycles, each with its unique characteristics and functions. Two key sleep phases are non-rapid eye movement (NREM) and  rapid eye movement (REM).
NREM Sleep
NREM sleep comprises four progressive stages that seamlessly merge:
1.3K
Hormonal Regulation01:33

Hormonal Regulation

The renin-aldosterone system is an endocrine system which guides the renal absorption of water and electrolytes, thus managing blood pressure and osmoregulation. Activation of the system begins in the kidneys with a small cluster of cells adjacent to the afferent and efferent blood vessels of the renal corpuscle. As the nephrons are filtering blood, juxtaglomerular cells monitor blood pressure. If they detect a decrease in pressure, they release the hormone renin into the bloodstream.
33.1K