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Mouse Models of Cancer Study

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Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
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Related Experiment Video

Updated: Jun 23, 2025

Bioluminescence Imaging of an Immunocompetent Animal Model for Glioblastoma

Bioluminescence Imaging of an Immunocompetent Animal Model for Glioblastoma

Published on: January 15, 2016

Optimization, Characterization, and Comparison of Two Luciferase-Expressing Mouse Glioblastoma Models.

Louis T Rodgers¹, Julia A Schulz Pauly¹, Bryan J Maloney²

¹Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY 40536, USA.

|June 19, 2024

Summary

Optimizing glioblastoma (GBM) models is crucial for cancer research. This study evaluates luciferase-expressing GBM models, highlighting engraftment differences and tumor characteristics for improved preclinical study design.

Keywords:

GL261 Red-FLuc TRP-mCherry-FLuc artificial intelligence bioluminescence imaging glioblastoma luciferase preclinical

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Last Updated: Jun 23, 2025

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Intracranial Implantation with Subsequent 3D In Vivo Bioluminescent Imaging of Murine Gliomas

Intracranial Implantation with Subsequent 3D In Vivo Bioluminescent Imaging of Murine Gliomas

Published on: November 6, 2011

Area of Science:

Oncology
Preclinical Cancer Research
In Vivo Imaging

Background:

Glioblastoma (GBM) is an aggressive brain cancer.
Orthotopic models like GL261 and TRP are used to study GBM.
Bioluminescence imaging (BLI) tracks tumor growth but luciferase expression can cause immune responses.

Purpose of the Study:

To optimize engraftment of luciferase-expressing GBM models.
To characterize tumor growth and invasiveness.
To evaluate imaging and analysis techniques for GBM research.

Main Methods:

Engraftment of GL261 Red-FLuc and TRP-mCherry-FLuc GBM models in mice.
Immunohistochemistry and MRI for tumor characterization.
HALO AI analysis for tumor volume quantification.

Main Results:

GL261 Red-FLuc required immunocompromised mice for 100% engraftment.
TRP-mCherry-FLuc tumors showed invasive and necrotic characteristics.
HALO AI provided efficient and standardized tumor volume analysis.

Conclusions:

Tumor engraftment is a critical factor in luciferase-expressing GBM models.
Distinct tumor characteristics were observed between models.
Standardized analysis platforms enhance preclinical research reliability.