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Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion
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Type IX Collagen Turnover Is Altered in Patients with Solid Tumors
Helena Port1,2, Yi He1, Morten A Karsdal1
1Immunoscience, Nordic Bioscience, 2730 Herlev, Denmark.
Cancers
|June 19, 2024
Summary
Type IX collagen (PRO-C9) shows potential as a non-invasive biomarker for multiple solid tumors. Elevated PRO-C9 levels in patient serum may aid in cancer detection and understanding collagen
Area of Science:
- Biochemistry
- Oncology
- Biomarker Discovery
Background:
- The tumor microenvironment's extracellular matrix (ECM) contains collagens with unexplored biomarker potential.
- Type IX collagen, a fibril-associated collagen with interrupted triple helices (FACIT), is primarily found in cartilage and its role in cancer is unknown.
Purpose of the Study:
- To investigate the biomarker potential of type IX collagen in cancer.
- To develop and validate an immunoassay (PRO-C9) for serum assessment of type IX collagen.
Main Methods:
- Developed a PRO-C9 immunoassay for serum analysis.
- Measured PRO-C9 levels in 259 solid tumor patients and 73 healthy controls.
- Evaluated diagnostic utility using receiver operating characteristic (ROC) analysis.
Main Results:
- PRO-C9 levels were significantly elevated in patients with bladder, breast, colorectal, gastric, head and neck, lung, melanoma, ovarian, pancreatic, and renal tumors compared to healthy controls.
- PRO-C9 demonstrated diagnostic utility, discriminating between cancer patients and healthy individuals (AUC 0.58–0.86).
Conclusions:
- Type IX collagen turnover is altered in patients with solid tumors.
- PRO-C9 is a feasible non-invasive serum biomarker for multiple cancer types.
- PRO-C9 may help elucidate the biology of FACITs in cancer.
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