Comprehensive Analysis Reveals the Difference in Volatile Oil between Bupleurum marginatum var. stenophyllum (Wolff) Shan et Y. Li and the Other Four Medicinal Bupleurum Species

Yuzhi Ma ¹, Xinwei Guo ¹, Peiling Wu ¹

⁰Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education and National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.

Molecules (basel, Switzerland) +

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June 19, 2024

View abstract on PubMed

Summary

Bupleurum marginatum var. stenophyllum possesses unique volatile oil profiles, differing significantly from other Bupleurum species. Its distinct chemical and anatomical structures support its potential as a valuable medicinal plant resource.

Area Of Science

Phytochemistry
Pharmacognosy
Plant Anatomy

Background

Volatile oils in Bupleuri Radix are traditional antipyretics.
Bupleurum marginatum var. stenophyllum is noted for high saikosaponin and volatile oil content.
Its utility as an alternative medicinal resource requires further investigation.

Purpose Of The Study

To systematically compare volatile oil components of B. marginatum var. stenophyllum with four official Bupleurum species.
To analyze chemical, molecular, bionics, and anatomical differences.
To evaluate the viability of B. marginatum var. stenophyllum as a medicinal resource.

Main Methods

Gas Chromatography-Mass Spectrometry (GC-MS) for volatile compound analysis.
Electronic-nose (e-nose) analysis for olfactory profiling.
Transcriptome analysis to identify differentially expressed genes (DEGs) in metabolic pathways.
Microscopic observation with staining for anatomical structure analysis.

Main Results

962 compounds identified in dried roots; B. marginatum var. stenophyllum showed significant differences in hydrocarbons, esters, and ketones.
Chemical profile differences were consistent between dried and fresh roots, corroborated by e-nose data.
Transcriptome analysis revealed numerous DEGs in the monoterpene synthesis pathway.
Microscopic analysis indicated a higher proportion of phloem tissue in B. marginatum var. stenophyllum, the site of volatile oil production.

Conclusions

Bupleurum marginatum var. stenophyllum exhibits a distinct chemical and anatomical profile compared to other Bupleurum species.
The unique characteristics, particularly the phloem structure and volatile oil composition, highlight its potential as a valuable resource.
Further research and development are warranted for the judicious utilization of this Bupleurum species.

Keywords:

Bupleurum GC-MS e-nose phytochemistry volatile oil