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Biomedical And Clinical Sciences
Oncology And Carcinogenesis
Predictive And Prognostic Markers
Investigating The Hepatoprotective Properties Of Mulberry Leaf Flavonoids Against Oxidative Stress In Hepg2 Cells.

Home
Research Domains
Biomedical And Clinical Sciences
Oncology And Carcinogenesis
Predictive And Prognostic Markers
Investigating The Hepatoprotective Properties Of Mulberry Leaf Flavonoids Against Oxidative Stress In Hepg2 Cells.

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Investigating the Hepatoprotective Properties of Mulberry Leaf Flavonoids against Oxidative Stress in HepG2 Cells.

Qinhua Zheng^1,2, Ke Feng³, Wenting Zhong^1,2

¹College of Life Science, Zhuhai College of Science and Technology, Zhuhai 519041, China.

Molecules (Basel, Switzerland)

|June 19, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Mulberry leaf flavonoids (MLF) protect liver cells (HepG2) from oxidative damage caused by hydrogen peroxide. MLF reduces harmful reactive oxygen species and preserves cell integrity, indicating potential as a hepatoprotective agent.

Keywords:

HepG2 cells antioxidant activity mulberry leaf flavonoids oxidative stress

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Area of Science:

Biochemistry
Cell Biology
Pharmacology

Background:

Oxidative stress is a key factor in aging and disease.
Antioxidants, particularly flavonoids from functional foods, show promise against free radical damage.
Mulberry leaf flavonoids (MLF) are investigated for their protective potential.

Purpose of the Study:

To evaluate the protective effects of MLF against hydrogen peroxide (H₂O₂)-induced oxidative damage in HepG2 cells.
To assess MLF's impact on cellular morphology and key oxidative stress markers.

Main Methods:

HepG2 cells were treated with varying concentrations of MLF (62.5-500 μg/mL) and H₂O₂ (750 μM).
Assessed cellular morphology, reactive oxygen species (ROS) production, mitochondrial membrane potential, and leakage of LDH and ALT.

Analyzed malondialdehyde (MDA) levels, antioxidant enzyme activity, apoptosis-related proteins, and DNA damage.

Main Results:

MLF prevented H₂O₂-induced cell shape changes and significantly reduced ROS levels.
MLF restored mitochondrial membrane potential and decreased LDH and ALT leakage.
MLF lowered MDA content, modulated antioxidant enzymes, and attenuated DNA damage in HepG2 cells.

Conclusions:

Mulberry leaf flavonoids demonstrate significant hepatoprotective effects against oxidative stress in HepG2 cells.
MLF mitigates oxidative damage by reducing ROS, preserving mitochondrial function, and protecting cellular components.
These findings support the potential therapeutic application of MLF as a natural antioxidant for liver health.