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Colicin E3 and its immunity genes.

H Masaki, T Ohta

    Journal of Molecular Biology
    |March 20, 1985
    PubMed
    Summary
    This summary is machine-generated.

    The lexA protein regulates colicin E3 expression. Protein B provides immunity to colicin E3, with its expression dependent on recA function and occurring in two modes.

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Microbiology

    Background:

    • Plasmid ColE3-CA38 DNA segment analysis.
    • Identification of promoter-operator regions, genes A, B, and H.

    Purpose of the Study:

    • Determine the nucleotide sequence of the cloned DNA segment.
    • Investigate the regulation of colicin E3 expression and immunity.

    Main Methods:

    • DNA cloning into pBR328.
    • Nucleotide sequencing.
    • Deletion mapping of the immunity gene.
    • Recloning of gene B.

    Main Results:

    • Identified 'SOS boxes' suggesting lexA protein regulation.
    • Genes A and B face the same direction with a 9-nucleotide intergenic space.

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  • Protein B confirmed as the colicin E3 immunity substance.
  • E3 immunity expression partially depends on recA function.

    • Conclusions:

    • Colicin E3 expression is directly regulated by the lexA protein.
    • Two modes of E3 immunity expression: constitutive (low protein B) and SOS-induced (high protein B).
    • Protein B protects host cells from colicin E3 inactivation.