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Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy FSM
Published on: August 5, 2009
Spatially Multiplexed Speckle on 1D Sensors for High-Speed 2D Sensing Applications
Ricardo Rubio-Oliver1, Martin Sanz1, Michael Sigalov2
1Departamento de Óptica y de Optometría y Ciencias de la Visión, Facultad de Física, Universidad de Valencia, C/Doctor Moliner 50, 46100 Burjassot, Spain.
Abstract:
Speckle pattern-based remote vibration monitoring has recently become increasingly valuable in industrial, commercial, and medical applications. The dynamic and random nature of speckle patterns offers practical applications for imaging and measurement systems. The speckle pattern is an interference pattern generated by light scattered from a rough surface onto a remote plane. It is typically sensed using area scan cameras (2D), which are limited to framerates of 2-4 kHz and can only capture a small region of interest (ROI). In this work, we propose a technique that enables the capture of synthetic 2D speckle patterns using a 1D high-acquisition-rate sensor and a diffractive optical element (DOE) to produce image replicas. The multiple replicas are scanned by the 1D sensor simultaneously at different spatial positions. This method provides an ability to sense remote vibrations in all directions, contrary to the case with a simple 1D sensing system.

