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Related Experiment Video

Updated: Jun 23, 2025

Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells
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Clinical Microfluidic Chip Platform for the Isolation of Versatile Circulating Tumor Cells

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A High-Throughput Circular Tumor Cell Sorting Chip with Trapezoidal Cross Section.

Shijie Lu1,2, Ding Ma2,3, Xianqiang Mi1,2,3

  • 1School of Microelectronics, Shanghai University, 20 Chengzhong Road, Shanghai 201899, China.

Sensors (Basel, Switzerland)
|June 19, 2024
PubMed
Summary

This study introduces a novel spiral microfluidic chip for efficient, label-free isolation of circulating tumor cells (CTCs) from blood. The device demonstrates high purity and efficiency, paving the way for improved early cancer diagnosis.

Keywords:
CTChigh throughputmicrofluidicsorting

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Area of Science:

  • Biomedical Engineering
  • Oncology
  • Microfluidics

Background:

  • Circulating tumor cells (CTCs) are vital biomarkers for early cancer detection and prognosis.
  • Traditional methods for CTC isolation are often inefficient and lack accuracy.
  • A need exists for advanced techniques to isolate CTCs from peripheral blood.

Purpose of the Study:

  • To develop and validate a novel spiral microfluidic chip for rapid, label-free enrichment of CTCs.
  • To assess the chip's performance in separating particles of various sizes and simulating CTCs.
  • To evaluate the chip's efficiency in processing whole blood samples for clinical applications.

Main Methods:

  • Fabrication of a spiral microfluidic chip with an asymmetric trapezoidal cross-section using 3D printing and micro-nanotechnology.
  • Systematic optimization of flow rate, channel width, and particle concentration for device performance.
  • Validation using polystyrene particles (6, 15, and 25 μm) and simulated CTCs (A549 cells) in diluted whole blood.

Main Results:

  • High separation efficiency (98.3%) and purity (96.4%) achieved for 25 μm particles.
  • Successful enrichment of CTCs from 5 mL of diluted whole blood.
  • Achieved 88.9% separation efficiency and 96.4% purity for A549 cells at a throughput of 1400 μL/min.

Conclusions:

  • The developed spiral microfluidic chip offers an efficient and high-throughput method for label-free CTC enrichment.
  • This technology holds significant potential for early cancer diagnosis and automated clinical analysis.
  • The device enables effective recovery of CTCs from whole blood, advancing cancer research and diagnostics.