Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Depicting Primate-Like Granular Dorsolateral Prefrontal Cortex in the Chinese Tree Shrew.

eNeuro·2024
Same author

Application of long read sequencing in rare diseases: The longer, the better?

European journal of medical genetics·2024
Same author

A rabies virus-based toolkit for efficient retrograde labeling and monosynaptic tracing.

Neural regeneration research·2023
Same author

Complement Receptor 3 Pathway and NMDA Receptor 2B Subunit Involve Neuropathic Pain Associated with Spinal Cord Injury.

Journal of pain research·2022
Same author

A new anterograde trans-synaptic tracer based on Sindbis virus.

Neural regeneration research·2022
Same author

Anatomical Evidence for the Neural Connection from the Emotional Brain to Autonomic Innervation in the Anterior Chamber Structures of the Eye.

Current medical science·2022

Related Experiment Video

Updated: Jun 23, 2025

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants
09:20

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants

Published on: October 18, 2022

4.5K

Engineered AAV13 variants with enhanced transduction and confined spread.

Neng-Song Luo1, Yu-Xiang Cai1, Zeng-Peng Han2,3,4,5

  • 1Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, Hubei 430074, China.

Zoological Research
|June 19, 2024
PubMed
Summary

Engineered adeno-associated virus 13 (AAV13) variants show improved gene delivery to the central nervous system (CNS). Modifications enhance transduction efficiency for precise brain region targeting in research and gene therapy.

Keywords:
AAV13-585-7m8AAV13-587-7m8AAV13-YFAdeno-associated virus 13transduction efficiency

More Related Videos

Engineering and Evolution of Synthetic Adeno-Associated Virus AAV Gene Therapy Vectors via DNA Family Shuffling
21:55

Engineering and Evolution of Synthetic Adeno-Associated Virus AAV Gene Therapy Vectors via DNA Family Shuffling

Published on: April 2, 2012

28.5K
Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors
09:21

Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors

Published on: January 29, 2019

36.3K

Related Experiment Videos

Last Updated: Jun 23, 2025

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants
09:20

Isolation of Next-Generation Gene Therapy Vectors through Engineering, Barcoding, and Screening of Adeno-Associated Virus AAV Capsid Variants

Published on: October 18, 2022

4.5K
Engineering and Evolution of Synthetic Adeno-Associated Virus AAV Gene Therapy Vectors via DNA Family Shuffling
21:55

Engineering and Evolution of Synthetic Adeno-Associated Virus AAV Gene Therapy Vectors via DNA Family Shuffling

Published on: April 2, 2012

28.5K
Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors
09:21

Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors

Published on: January 29, 2019

36.3K

Area of Science:

  • Neuroscience
  • Gene Therapy
  • Molecular Biology

Background:

  • Precise targeting of the central nervous system (CNS) is vital for brain disease research and gene therapy.
  • Adeno-associated virus 13 (AAV13) offers restricted diffusion for targeting small brain regions but has low gene expression.
  • Enhancing AAV13 transduction efficiency is critical for effective CNS applications.

Purpose of the Study:

  • To engineer modified adeno-associated virus 13 (AAV13) capsid proteins to improve transduction efficiency.
  • To evaluate the infectivity and CNS targeting capabilities of novel AAV13 variants.

Main Methods:

  • Constructed AAV13-YF by mutating surface tyrosine to phenylalanine.
  • Inserted the 7m8 peptide into AAV13 capsid positions 587/588 and 585/586, creating AAV13-587-7m8 and AAV13-585-7m8.
  • Assessed in vitro infectivity in HEK293T cells and in vivo CNS infection in C57BL/6 mice.

Main Results:

  • AAV13-YF demonstrated superior in vitro infectivity compared to wild-type AAV13.
  • AAV13-587-7m8 and AAV13-585-7m8 exhibited enhanced CNS infection capabilities in mice.
  • AAV13-587-7m8 maintained a limited spread range within the CNS.

Conclusions:

  • Engineered AAV13 variants, including AAV13-YF, AAV13-587-7m8, and AAV13-585-7m8, show enhanced transduction efficiency.
  • These modified AAV13 vectors hold significant potential for precise gene delivery in neuroscience research and gene therapy for brain diseases.