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Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...

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Updated: Jun 23, 2025

Generation of Mice Derived from Induced Pluripotent Stem Cells

Generation of Mice Derived from Induced Pluripotent Stem Cells

Published on: November 29, 2012

A PLURIPOTENT STEM CELL PLATFORM FOR IN VITRO SYSTEMS GENETICS STUDIES OF MOUSE DEVELOPMENT.

Rachel A Glenn^1,2,3, Stephanie C Do^1,2, Karthik Guruvayurappan⁴

¹Developmental Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, USA.

Biorxiv : the Preprint Server for Biology

|June 19, 2024

Summary

This study introduces new pluripotent stem cell (PSC) lines and methods for studying genetic variation

Keywords:

complex trait genetics developmental biology genetics genomics pluripotent stem cells

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Last Updated: Jun 23, 2025

Generation of Mice Derived from Induced Pluripotent Stem Cells

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Published on: November 29, 2012

Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm

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Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro

Generation of Aggregates of Mouse Embryonic Stem Cells that Show Symmetry Breaking, Polarization and Emergent Collective Behaviour In Vitro

Published on: November 24, 2015

Area of Science:

Stem cell biology
Genetics
Developmental biology

Background:

Pluripotent stem cells (PSCs) from diverse individuals offer insights into genetic variation's impact on development.
Understanding genetic contributions to cell and tissue development is crucial.

Purpose of the Study:

To establish PSC lines and methods for modeling embryonic development in genetically diverse mice.
To characterize the role of genetic variation in gene regulation and differentiation.
To develop scalable approaches for genetic screens.

Main Methods:

Generation and maintenance of pluripotent stem cell (PSC) lines, including epiblast stem cells (EpiSCs).
Creation of a genetic reference panel of Diversity Outbred PSCs (n=230) using pooled in vitro fertilization.
Development of pooled culture techniques for EpiSCs ('cell villages').

Main Results:

Demonstrated stable maintenance of primed pluripotent stem cells (epiblast stem cells - EpiSCs) across diverse genetic backgrounds.
Quantified the impact of genetic variation on gene regulation and directed differentiation.
Established pooled EpiSC culture as a feasible method for large-scale genetic screens.

Conclusions:

New PSC lines and experimental approaches enable the study of natural genetic variation in embryonic development.
Pooled culture of Diversity Outbred EpiSCs facilitates efficient forward genetic screens.
This work complements human genetics and stem cell biology efforts to model genetic variation and disease risk.