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Lysis protein encoded by plasmid ColA-CA31. Gene sequence and export.

D Cavard, R Lloubès, J Morlon

    Molecular & General Genetics : MGG
    |January 1, 1985
    PubMed
    Summary

    Researchers identified the cal gene in Escherichia coli, crucial for releasing colicin A. This gene

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    Area of Science:

    • Bacteriology
    • Molecular Biology
    • Genetics

    Background:

    • Colicin A is a bacteriocin produced by Escherichia coli.
    • The release mechanism of colicin A from E. coli cells is not fully understood.
    • Related plasmids encode proteins homologous to the identified cal protein.

    Purpose of the Study:

    • To identify and characterize the gene responsible for colicin A release in E. coli.
    • To determine the genetic location and transcriptional direction of the cal gene.
    • To elucidate the structure and function of the cal protein.

    Main Methods:

    • Transposon mutagenesis to identify the cal gene.
    • DNA sequencing to determine the cal gene sequence.
    • Radioactive amino acid labeling and SDS-PAGE to identify and analyze the cal protein.
    • Plasmid mapping to determine gene location.

    Main Results:

    • The cal gene was identified adjacent to the colicin A immunity gene (cai) and downstream of the colicin A structural gene (caa).
    • The cal gene sequence predicts a protein with a signal sequence-like N-terminus and hydrophobic C-terminus.
    • The cal protein is homologous to lytic proteins from related plasmids and is co-induced with colicin A.
    • The cal protein is processed, losing its N-terminal signal region, and released with colicin A.

    Conclusions:

    • The cal gene encodes a protein essential for colicin A release from E. coli.
    • The cal protein shares structural and functional similarities with other plasmid-encoded lytic proteins.
    • The cal protein likely acts as a bacteriocin release protein, facilitating colicin A secretion.

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