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Related Experiment Video

Updated: Jun 12, 2026

Fragility Assessment of Bovine Cortical Bone Using Scratch Tests

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Published on: November 30, 2017

In vitro Models for Predicting Bioadhesion Fracture Strength to Ex Vivo Animal Buccal Tissue.

Valeria Solomianski Rahamim¹, Dhaval Patel¹, Eliyahu Drori¹

¹Department of Chemical and Biotechnology Engineering, Ariel University, Kyriat-ha-Mada 3, Ariel, 4070000, Israel.

Small (Weinheim an Der Bergstrasse, Germany)

|June 19, 2024

Summary

A new cell-based system effectively replaces porcine tissues for measuring buccal bioadhesion. This in vitro model accurately predicts bioadhesion, supporting the 3Rs principle and advancing drug delivery systems.

Keywords:

TR146 cells bioadhesion buccal drug delivery in vitro models mucoadhesion

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Published on: December 20, 2024

Area of Science:

Biomaterials Science
Pharmacology
In Vitro Models

Background:

The 3Rs principle (Replacement, Reduction, and Refinement) necessitates alternatives to animal testing.
Porcine buccal and esophageal tissues (PBT and PET) are traditionally used to study buccal bioadhesion.
A knowledge gap exists regarding the bioadhesion properties of PBT and PET.

Purpose of the Study:

To develop and validate a cell-based in vitro system for measuring buccal bioadhesion.
To replace the use of PBT and PET in bioadhesion studies.
To compare the bioadhesion performance of standard hydrogels using both in vitro and ex vivo models.

Main Methods:

Development of two in vitro models using the human buccal epithelial cell line TR146 (Model I without mucous layer, Model II with mucous layer).
Evaluation of bioadhesion between hydrogels (alginate, chitosan, gelatin) and the in vitro/ex vivo models.
Comparison of bioadhesion data generated from in vitro models with ex vivo PBT and PET data.

Main Results:

Both in vitro and ex vivo models demonstrated increased bioadhesion with higher applied force and contact time.
Model I showed bioadhesion values for alginate, chitosan, and gelatin comparable to those obtained with PBT.
Contact time and applied force influenced PBT and PET bioadhesion similarly, with PET exhibiting higher values.

Conclusions:

The developed in vitro Model I can successfully replace PBT for buccal bioadhesion measurements.
This cell-based system supports the 3Rs principle by minimizing animal tissue usage.
The validated in vitro model can be integrated into the design of bioadhesive drug delivery systems (DDS).