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Mutascreen, an automated bacterial mutagenicity assay.

K Falck, P Partanen, M Sorsa

Mutation Research

|June 1, 1985

Summary

Mutascreen offers automated bacterial mutagenicity testing, mirroring the Ames test but with robotic precision. This automated system accurately identifies mutagens, showing potential for efficient genotoxicity screening.

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Area of Science:

Toxicology
Genetics
Biotechnology

Background:

Bacterial reverse-mutation assays, like the Ames test, are standard for mutagenicity testing.
Conventional methods can be labor-intensive and time-consuming.

Purpose of the Study:

To evaluate the Mutascreen automated instrument for bacterial mutagenicity testing.
To compare the performance of Mutascreen with the conventional plate assay.

Main Methods:

Mutascreen utilizes a computer-controlled robot for dispensing and a vertical-pathway photometer for monitoring growth in 400-microliter wells over 24 hours.
Assay principles are similar to the Ames test, analyzing changes in bacterial growth patterns and turbidity.
36 chemicals, including known carcinogens and noncarcinogens, were tested using both Mutascreen and conventional plate assays.

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Main Results:

Mutascreen successfully identified mutagens among the tested chemicals.
Results from Mutascreen were quantitatively similar to the conventional plate assay.
Automated monitoring avoided sedimentation issues common in other methods.

Conclusions:

The Mutascreen system demonstrates potential usefulness for automated bacterial mutagenicity testing.
Automation offers an efficient and accurate alternative for genotoxicity screening.
The instrument provides reliable data comparable to established methods.