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New mutations affecting induced mutagenesis in yeast.

C W Lawrence, B R Krauss, R B Christensen

    Mutation Research
    |June 1, 1985
    PubMed
    Summary
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    Researchers screened baker's yeast for mutations affecting DNA repair, identifying new genes involved in mutagenesis. This study expands our understanding of DNA repair mechanisms and mutation induction in Saccharomyces cerevisiae.

    Area of Science:

    • * Molecular Biology
    • * Genetics
    • * Yeast Genetics

    Background:

    • * Previous studies identified mutations impairing induced mutagenesis primarily using assays detecting base-pair substitutions.
    • * This limited the scope of discovered mutations, potentially overlooking those affecting other mutation types.

    Purpose of the Study:

    • * To identify mutations affecting induced mutagenesis in Saccharomyces cerevisiae using a method unbiased towards base-pair substitutions.
    • * To discover novel genes involved in DNA repair and mutagenesis pathways.

    Main Methods:

    • * Screening of 11,366 mutant clones of Saccharomyces cerevisiae.
    • * Selection for UV-induced reversion of the frameshift allele his4-38.
    • * Complementation and recombination tests to identify and map mutations.

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    Main Results:

    • * Identified 10 mutants with significantly reduced yields of revertants.
    • * Six mutants carried known mutations at REV1 and REV3 loci.
    • * Four mutants defined three new genes: REV4 (2 mutations), REV5, and REV6.
    • * rev4 and rev5 mutations showed limited deficiency in induced mutagenesis.
    • * rev6 mutants exhibited general deficiency, UV sensitivity, and increased spontaneous mutation rate.
    • * REV5 gene mapped near CYC1 on chromosome X.

    Conclusions:

    • * Discovery of three new genes (REV4, REV5, REV6) involved in DNA repair and mutagenesis.
    • * REV6 appears directly involved in mutation induction due to its pleiotropic effects.
    • * REV4 and REV5 gene functions are likely indirectly involved in mutagenesis.
    • * This research provides new insights into the complex mechanisms of DNA repair and mutation induction.