Pengzhenrongella frigida sp. nov., isolated from a glacier

A Gram-stain-positive, rod-shaped bacterium, designated as HLT2-17^T, was isolated from soil sample taken from the Hailuogou glacier in Sichuan province, PR China. Strain HLT2-17^T was capable of growing at 4-25°C and in NaCl concentrations ranging from 0 to 2% (w/v). The highest level of 16S rRNA gene sequence similarity was observed with Pengzhenrongella phosphoraccumulans M0-14^T (98.3 %) and Pengzhenrongella sicca LRZ-2^T (98.2 %). The average nucleotide identity and digital DNA-DNA hybridization values between strain HLT2-17^T and its closest relatives, P. phosphoraccumulans M0-14^T and P. sicca LRZ-2^T, were 80.0-84.0 % and 23.3-27.7 %, respectively. Phylogenomic analysis indicated that strain HLT2-17^T clustered together with strains P. phosphoraccumulans M0-14^T and P. sicca LRZ-2^T. Strain HLT2-17^T contained C_16 : 0 and anteiso-C_15 : 0 as the major fatty acids, and MK-9(H₄) as the menaquinone. Therefore, based on a polyphasic approach, we propose that strain HLT2-17^T (=CGMCC 1.11116^T= NBRC 110443^T) represents a novel species of the genus Pengzhenrongella and suggest the name Pengzhenrongella frigida sp. nov.