Stable detection of diazinon residues in vegetables by an electrochemiluminescent aptasensor based on the in-situ production of H₂O₂ from dual-catalytic glucose

Jingcheng Huang ¹, Mei Zhang ¹, Xue Huang ²

⁰School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, Shandong, 255049, China.

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June 19, 2024

View abstract on PubMed

Summary

A new electrochemiluminescence aptasensor offers stable detection of diazinon (DZN) residues in vegetables. This method utilizes dual-catalytic glucose for in-situ hydrogen peroxide production, enhancing food safety analysis.

Area Of Science

Analytical Chemistry
Biosensors
Food Safety

Background

Stable detection of diazinon (DZN) residues in vegetables is crucial for food safety.
Conventional methods face challenges due to the instability of hydrogen peroxide (H₂O₂).

Purpose Of The Study

To develop a stable electrochemiluminescence (ECL) aptasensor for DZN detection in vegetables.
To address the instability of H₂O₂ in luminescence systems through in-situ generation.

Main Methods

Fabrication of a dual-catalytic system using MWCNTs@MB and Cu₂O@AuNPs for in-situ H₂O₂ production from glucose.
Immobilization of aptamers on the electrode surface via Au-S bonds with Cu₂O@AuNPs.
Construction of an ECL aptasensor for DZN detection.

Main Results

The aptasensor demonstrated good linearity in the range of 1.00 pg mL⁻¹ to 1.00 μg mL⁻¹.
A low limit of detection (LOD) of 0.39 pg mL⁻¹ (S/N = 3) was achieved.
The in-situ H₂O₂ generation effectively stabilized the detection system.

Conclusions

The developed ECL aptasensor provides an accurate and stable method for DZN residue detection in vegetables.
This approach significantly contributes to ensuring food safety and assessing environmental risks associated with DZN.

Keywords:

Aptasensor Diazinon Dual catalysts Electrochemiluminescence In-situ generation of H(2)O(2)