Related Experiment Video

Updated: Jun 23, 2025

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
08:07

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Published on: July 26, 2019

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PIK3IP1: structure, aberration, function, and regulation in diseases

Yingjie Jia1, Pengxing He1, Xubin Ma1

  • 1Key Laboratory of Advanced Drug Preparation Technologies, Ministry of Education, School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, China.

European Journal of Pharmacology
|June 19, 2024
PubMed

Insights

Phosphatidylinositol-3-kinase interacting protein 1 (PIK3IP1) is a novel negative regulator of the PI3K pathway. Low PIK3IP1 expression correlates with cancer and autoimmune diseases, impacting cell proliferation, survival, and immune function.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Cancer Research

Background:

  • The Phosphoinositide 3-kinase (PI3K) pathway is crucial for cellular functions and frequently dysregulated in cancer.
  • Negative regulators are essential for controlling PI3K pathway overactivation.
  • Phosphatidylinositol-3-kinase interacting protein 1 (PIK3IP1) is a newly identified transmembrane protein that negatively regulates PI3K activity.

Purpose of the Study:

  • To review the structure, biological functions, and regulatory mechanisms of PIK3IP1.
  • To integrate findings on PIK3IP1 in various diseases, particularly cancer and autoimmune disorders.
  • To highlight the role of PIK3IP1 as a negative regulator of the PI3K pathway.

Main Methods:

  • Literature review integrating existing research findings.
  • Analysis of PIK3IP1's interaction with the p110 catalytic subunit of PI3K.
  • Examination of PIK3IP1 expression patterns in different disease contexts.

Main Results:

  • PIK3IP1 directly binds to the p110 catalytic subunit, inhibiting PI3K activity.
  • Low PIK3IP1 expression is frequently observed in tumors and autoimmune diseases.
  • PIK3IP1 inhibits tumor cell proliferation and survival, and maintains immune cell quiescence.

Conclusions:

  • PIK3IP1 plays a critical role in suppressing PI3K pathway overactivation.
  • Dysregulation of PIK3IP1 is linked to the pathogenesis of cancer and autoimmune diseases.
  • Understanding PIK3IP1's regulatory mechanisms is vital for therapeutic strategies in these conditions.
Keywords:
Autoimmune diseaseCancer progressionInflammatory responsePI3K pathwayPIK3IP1

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