Related Experiment Video
Updated: Jun 14, 2026

Vibrodissociation of Neurons from Rodent Brain Slices to Study Synaptic Transmission and Image Presynaptic Terminals
Published on: May 25, 2011
High-throughput volumetric mapping of synaptic transmission
Wei Chen1,2, Xinxin Ge3,4, Qinrong Zhang1
1Department of Physics, University of California, Berkeley, CA, USA.
Abstract:
Volumetric imaging of synaptic transmission in vivo requires high spatial and high temporal resolution. Shaping the wavefront of two-photon fluorescence excitation light, we developed Bessel-droplet foci for high-contrast and high-resolution volumetric imaging of synapses. Applying our method to imaging glutamate release, we demonstrated high-throughput mapping of excitatory inputs at >1,000 synapses per volume and >500 dendritic spines per neuron in vivo and unveiled previously unseen features of functional synaptic organization in the mouse primary visual cortex.
Related Concept Videos
Transmission Electron Microscopy
Radiation: Applications
The average...
Momentum And Radiation Pressure
The Maximum Power Transfer Theorem
The load connected draws the current, and the circuit delivers the power to the load. The alternating current flowing through the load is determined using the rectangular form of voltages, currents, network impedance, and load impedance. The average power delivered to the load is obtained from the product of the square of current and load resistance.
Carrier-Mediated Transport
Active transport involves two types of membrane-spanning transporters: uptake and efflux. Uptake transporters are expressed in the small...

