Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

5.1K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
5.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neutrophil myeloperoxidase as a functional biomarker for RSV severity: implications for in vitro therapeutic screening.

Nature communications·2026
Same author

Innate immune responsiveness predicts enhanced cellular immunity and symptomatic disease after controlled human influenza infection.

Nature medicine·2026
Same author

Phase 2a Randomized Placebo-Controlled Human Challenge Trial of the RSV L-Protein Inhibitor S-337395 for Respiratory Syncytial Virus Infection.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2026
Same author

Donor-derived airway air-liquid interface model for high-throughput screening of antiviral combinations with concurrent analysis of antiviral efficacy and epithelial toxicity using ciliR.

ERJ open research·2026
Same author

Heterogeneity and dynamics of DENV-specific CD8 + T cells in dengue infection.

Nature communications·2026
Same author

NETosis associates with human TB lung tissue destruction and disease pathogenesis.

EMBO molecular medicine·2026

Related Experiment Video

Updated: Jun 23, 2025

Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2
08:41

Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2

Published on: November 5, 2021

2.7K

Human SARS-CoV-2 challenge uncovers local and systemic response dynamics.

Rik G H Lindeboom1,2, Kaylee B Worlock3, Lisa M Dratva4,5

  • 1Wellcome Sanger Institute, Wellcome Genome Campus, Cambridge, UK. r.lindeboom@nki.nl.

Nature
|June 19, 2024
PubMed
Summary

This study reveals early cellular immune responses to SARS-CoV-2 infection. Understanding these dynamics, including interferon response and T-cell activation, is key to developing strategies against COVID-19.

More Related Videos

Production of a SARS-CoV-2 Virus-Like-Particle System to Investigate Viral Life Cycles In Vitro
09:26

Production of a SARS-CoV-2 Virus-Like-Particle System to Investigate Viral Life Cycles In Vitro

Published on: June 6, 2025

120
Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses
05:49

Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses

Published on: November 21, 2023

1.7K

Related Experiment Videos

Last Updated: Jun 23, 2025

Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2
08:41

Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2

Published on: November 5, 2021

2.7K
Production of a SARS-CoV-2 Virus-Like-Particle System to Investigate Viral Life Cycles In Vitro
09:26

Production of a SARS-CoV-2 Virus-Like-Particle System to Investigate Viral Life Cycles In Vitro

Published on: June 6, 2025

120
Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses
05:49

Author Spotlight: Studying Host-Virus Interactions with Pseudotyped Viruses

Published on: November 21, 2023

1.7K

Area of Science:

  • Immunology
  • Virology
  • Genomics

Background:

  • The COVID-19 pandemic highlights gaps in understanding early cellular immunity to SARS-CoV-2.
  • Limited knowledge exists on the temporal dynamics of host responses during initial infection stages.

Purpose of the Study:

  • To temporally resolve abortive, transient, and sustained SARS-CoV-2 infections using single-cell multi-omics.
  • To identify dynamic cellular responses in epithelial and immune cells during early infection.
  • To investigate early immune responses associated with protection against sustained infection.

Main Methods:

  • Single-cell multi-omics profiling of nasopharyngeal swabs and blood from seronegative individuals challenged with SARS-CoV-2.
  • Temporal analysis of cell-type proportions and cellular response states.
  • Development and application of the Cell2TCR computational pipeline to identify antigen-responding T-cells.

Main Results:

  • Rapid changes in cell-type proportions and dynamic cellular responses were observed.
  • Interferon response in blood preceded nasopharyngeal response.
  • Early nasopharyngeal immune infiltration correlated with transient infection; later infiltration with sustained infection.
  • High HLA-DQA2 expression pre-inoculation was linked to protection against sustained infection.
  • Ciliated cells showed high permissiveness to viral replication, while T-cells and macrophages exhibited non-productive infection.
  • 54 T-cell states were identified, including clonally expanded T-cells with convergent SARS-CoV-2 motifs.

Conclusions:

  • Detailed time-series data provide a comprehensive view of epithelial and immune cell responses to SARS-CoV-2.
  • Early dynamic immune responses are associated with protection against sustained infection.
  • The Cell2TCR pipeline aids in identifying and clustering antigen-responding T-cells.