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Related Experiment Videos

Does the 'non-coding' strand code?

P M Sharp

    Nucleic Acids Research
    |February 25, 1985
    PubMed
    Summary
    This summary is machine-generated.

    This study investigated if DNA non-coding strands contain protein-coding sequences. Analysis revealed no significant evidence for this in bacteriophage T7, Bacillus, or yeast, suggesting non-coding strands primarily serve a complementary role.

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    Area of Science:

    • Genomics
    • Molecular Biology
    • Bioinformatics

    Background:

    • The non-coding DNA strand is traditionally viewed as complementary to the messenger RNA (mRNA) template.
    • There has been a hypothesis that the non-coding strand might also contain functional coding sequences.
    • Understanding the functional potential of both DNA strands is crucial in genomics.

    Purpose of the Study:

    • To investigate the hypothesis that DNA strands complementary to the coding strand contain in-phase coding sequences.
    • To analyze codon usage patterns on coding and non-coding DNA strands across different organisms.
    • To determine if the non-coding strand exhibits properties of a protein-encoding sequence.

    Main Methods:

    • Statistical analysis of codon usage patterns.

    Related Experiment Videos

  • Comparative analysis of gene sequences from bacteriophage T7, Bacillus, yeast, and Escherichia coli.
  • Assessment of correlations between codon usage on coding and non-coding strands.

    • Main Results:

    • No significant correlation between codon usage patterns on coding and non-coding strands was found in bacteriophage T7 genes.
    • Observed correlations in Bacillus and yeast genes were consistent with random synonymous codon usage.
    • A high correlation in E. coli genes was attributed to an excess of RNY codons, not protein-encoding potential.
    • A deficiency of specific codons (UUA, CUA, UCA) complementary to termination signals was noted in E. coli, possibly due to tRNA abundance.

    Conclusions:

    • The non-coding DNA strand generally exhibits properties expected of a sequence complementary to a coding strand.
    • There is no significant evidence that the non-coding strand encodes, or has encoded, proteins in the studied organisms.
    • Specific codon usage patterns in E. coli may be influenced by tRNA availability rather than dual coding functions.