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Updated: Jun 21, 2026
Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
Published on: June 9, 2020
Protocol for multimodal profiling of human kidneys with simultaneous high-throughput ATAC and RNA expression with
Haikuo Li1, Benjamin D Humphreys2
1Division of Nephrology, Department of Medicine, Washington University in St. Louis, St. Louis, MO 63105, USA.
None:
Simultaneous high-throughput ATAC and RNA expression with sequencing (SHARE-seq) profiles transcriptomics and chromatin accessibility in the same cells at high throughput. Here, we present a protocol for multimodal profiling of human kidneys with SHARE-seq. We describe steps for processing fixed nuclei for SHARE-seq split-pool barcoding and library preparation. We also detail how to determine the optimal working concentration of Tn5 transposase for transposition and tagmentation. This protocol allows researchers to generate large-scale single-cell multiomics data at low reagent cost. For complete details on the use and execution of this protocol, please refer to Li et al.1.
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