Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.0K
CRISPR01:59

CRISPR

50.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
50.5K
Homologous Recombination02:31

Homologous Recombination

50.4K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mako robot-assisted unicompartmental knee arthroplasty mitigates the impact of surgeon handedness.

Journal of robotic surgery·2026
Same author

Integrative Proteogenomic Characterization of Left-Sided and Right-Sided Colorectal Cancer.

MedComm·2026
Same author

Effects of anterior cruciate ligament resection and total knee arthroplasty on lower limb alignment and tibiofemoral rotation.

Journal of orthopaedics and traumatology : official journal of the Italian Society of Orthopaedics and Traumatology·2026
Same author

Avian coronaviruses induce inflammatory responses by activating p38/MAPK signaling and NLRP3/caspase-1 inflammasomes through sphingosine-1-phosphate receptor 1.

Veterinary research·2026
Same author

Tumors mimic the niche to inhibit neighboring stem cell differentiation.

eLife·2026
Same author

Ocular toxicity associated with antibody-drug conjugates in cancer therapy: a comprehensive review.

Frontiers in immunology·2026

Related Experiment Video

Updated: Jun 23, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.1K

The CRISPR-Cas system in molecular diagnostics.

Rao Song1, Zhongyi Chen2, Hongtao Xiao1

  • 1Department of Pharmacy, Sichuan Clinical Research Center for Cancer, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, Affiliated Cancer Hospital of University of Electronic Science and Technology of China, Chengdu 610041, China.

Clinica Chimica Acta; International Journal of Clinical Chemistry
|June 20, 2024
PubMed
Summary

The CRISPR-Cas system offers a promising alternative for molecular diagnostics, enabling rapid and sensitive disease detection without expensive equipment. This review highlights its potential to overcome limitations of current diagnostic methods.

Keywords:
BiosensorCOVID-19CRISPRCas12Cas13Cas9DetectionMolecular diagnosis

More Related Videos

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

2.6K
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

225

Related Experiment Videos

Last Updated: Jun 23, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.1K
Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

2.6K
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

225

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Medical Diagnostics

Background:

  • Current molecular diagnostic tools require expensive equipment and specialized personnel, hindering widespread disease diagnosis and epidemiological control.
  • The bacterial CRISPR-Cas system, a natural defense mechanism, involves targeted DNA/RNA cleavage mediated by Cas proteins and CRISPR RNA.
  • Recent advancements have revealed the CRISPR-Cas system's potential for revolutionizing molecular diagnostics.

Purpose of the Study:

  • To provide a comprehensive overview of the CRISPR-Cas system's recent research progress.
  • To focus on the application of the CRISPR-Cas system in the field of molecular diagnosis.
  • To highlight the advantages of CRISPR-Cas based diagnostics over conventional methods.

Main Methods:

  • Literature review of recent scientific publications on CRISPR-Cas systems.
  • Analysis of studies focusing on CRISPR-Cas applications in molecular detection.
  • Synthesis of information regarding the mechanism and diagnostic utility of CRISPR-Cas.

Main Results:

  • The CRISPR-Cas system demonstrates robust, sensitive, and rapid molecular detection capabilities.
  • CRISPR-Cas based diagnostics can potentially circumvent the need for expensive equipment and specialized operators.
  • The system's programmability and specificity make it highly adaptable for various diagnostic targets.

Conclusions:

  • The CRISPR-Cas system presents a significant advancement in molecular diagnostics, offering accessible and efficient disease detection.
  • Further research and development are expected to expand the clinical utility of CRISPR-Cas technology for global health.
  • CRISPR-Cas based diagnostics are poised to transform disease surveillance and patient management.