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Updated: Jun 23, 2025

Use of the Soft-agar Overlay Technique to Screen for Bacterially Produced Inhibitory Compounds
Published on: January 14, 2017
Chemical compounds from Osmunda japonica and their NO inhibitory activity
Xueshan Feng1, Min Cai1, Lanzhu Zhu1
1Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China.
Abstract:
Two new compounds, osmjapterpenoid A (1) and osmunjaponin A (2), along with twenty-six known compounds, were isolated from the roots and rhizomes of Osmunda japonica Thunb. The chemical structures of them were elaborated by extensive spectroscopic means, including 1D, 2D-NMR and HR-ESI-MS. Compound 1 is a diterpenoid derived from cembrane with a novel skeleton of 5/13 dicyclic ring system. The possible biogentic pathway of 1 was deduced. Compounds 3 and 26 exhibited moderate inhibition on lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 macrophages with IC50 value of 32.09 and 19.81 μM, respectively.
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