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Updated: Jun 23, 2025

Analyzing Satellite Cell Function During Skeletal Muscle Regeneration by Cardiotoxin Injury and Injection of Self-delivering siRNA In Vivo
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Dasatinib promotes muscle differentiation and disrupts normal muscle regeneration

Nanami Ishida1, Tamaki Kurosawa1, Momo Goto1

  • 1Laboratory of Veterinary Pharmacology, Department of Veterinary Medical Sciences, Graduate School of Agriculture and Life Sciences, Tokyo University, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.

International Journal of Medical Sciences
|June 21, 2024
PubMed

Insights

Dasatinib, used for leukemia, disrupts normal muscle regeneration by altering gene expression in myoblasts and muscle fibers. This tyrosine kinase inhibitor impacts skeletal muscle development and repair processes.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Muscle Physiology

Background:

  • Dasatinib is a second-generation tyrosine kinase inhibitor with a broad target spectrum.
  • Previous research suggests dasatinib may improve athletic performance and affect muscle diseases, but its impact on myoblasts is unknown.

Purpose of the Study:

  • To investigate the effects of dasatinib on skeletal muscle under normal and regenerating conditions.
  • To determine dasatinib's influence on C2C12 myoblast proliferation and fusion.

Main Methods:

  • Utilized C2C12 myoblasts to assess proliferation and fusion.
  • Administered dasatinib to a mouse model with CTX-induced muscle injury.
  • Analyzed gene expression of myogenic-related factors (Myod, Myog, Mymx).

Main Results:

  • Dasatinib suppressed C2C12 myoblast proliferation but promoted fusion.
  • Increased gene expression of myogenic regulatory factors during muscle regeneration.
  • Induced abnormally thin muscle fibers in a mouse model of muscle injury.

Conclusions:

  • Dasatinib alters the gene expression patterns of myogenic regulatory factors during muscle differentiation.
  • The drug disrupts normal skeletal muscle regeneration processes.
  • Consideration of dasatinib's skeletal muscle effects is crucial, especially during regeneration stages.
Keywords:
C2C12CTX injury modelDasatinibmuscle regeneration

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