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Updated: Jun 23, 2025

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin
Published on: March 31, 2011
Isolation, Characterization, and Utilization of Human Skin Basal and Suprabasal Epidermal Stem Cells
Luca Pontiggia1,2, Agnes S Klar1,2,3, Katarzyna Michalak-Micka1,2
1Tissue Biology Research Unit, Department of Pediatric Surgery, University Children's Hospital Zurich, Schlieren, Switzerland.
Abstract:
Studying human skin biology can aid in comprehending the pathophysiology of skin diseases and developing novel cell-based therapies, including tissue engineering approaches. This chapter provides a comprehensive guide of methods to determine human skin samples from the perspective of their cellular compositions. We describe as useful technique the histological analysis of tissue sections. We further illustrate the biological characterization of isolated and cultured basal and suprabasal interfollicular keratinocytes by cell sorting, cytospin immunostaining, colony forming efficiency, and long-term dermo-epidermal organotypic cultures.
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