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Published on: March 31, 2021
Protocol for the creation and characterization of SARS-CoV-2 variant testing panels using remnant clinical samples
Leda Bassit1, Heather B Bowers1, Morgan Greenleaf2
1The Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, GA, USA; Center for ViroScience and Cure, Laboratory of Biochemical Pharmacology, Department of Pediatrics, Emory University, Atlanta, GA, USA.
Abstract:
The emergence of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Alpha variant in 2020 demonstrated the need for reanalysis of diagnostic tests to ensure detection of emerging variants. Here, we present a protocol for creating and characterizing SARS-CoV-2 variant testing panels using remnant clinical samples for diagnostic assay testing. We describe steps for characterizing SARS-CoV-2 remnant clinical samples and preparing them into pools and their use in preparing varying quantities of virus. We then detail procedures for verifying variant detection using the resulting sample panel. For complete details on the use and execution of this protocol, please refer to Rao et al.1,2.
